Our collaborators
Signalling Factory
Signalling Factory
Labs listed in alphabetical order.
https://www.uniklinik-freiburg.de/pathologie/forschung/ag-bronsert.html
Methods: Cloning, Stable cell line generation (over-expression, immortalization)
Publication: In preparation
https://www.uniklinik-freiburg.de/humangenetik/aktuelles.html
Methods: Cloning, Protein expression and purification, Activity measurement
Publications: A Monoallelic Two-Hit Mechanism in PLCD1 Explains the Genetic Pathogenesis of Hereditary Trichilemmal Cyst Formation.
Hörer S, Marrakchi S, Radner FPW, Zolles G, Heinz L, Eichmann TO, Has C, Salavei P, Mahfoudh N, Turki H, Zimmer AD, Fischer J.
J Invest Dermatol. 2019 Oct;139(10):2154-2163.e5.
Mutations in SULT2B1 Cause Autosomal-Recessive Congenital Ichthyosis in Humans.
Heinz L, Kim GJ, Marrakchi S, Christiansen J, Turki H, Rauschendorf MA, Lathrop M, Hausser I, Zimmer AD, Fischer J.
Am J Hum Genet. 2017 Jun 1;100(6):926-939. doi: 10.1016/j.ajhg.2017.05.007.
https://www.uniklinik-freiburg.de/cci/forschung/bodo-grimbacher.html
Methods Establishing cell sorting method
http://www.plantphotobiology.org/
Methods Establishing cell sorting method
http://www.biochemie.uni-freiburg.de/de/ag/hunte
Methods Large scale cell culture (transfection), Stable cell line generation (over-expression and knock-out cell line), Confocal microscopy
Publication: In progress
https://www.bioss.uni-freiburg.de/groups/intergrated-signalling-studies/home/
Methods: Confocal Microscopy
Publication: Dissecting the sequence determinants for dephosphorylation by the catalytic subunits of phosphatases PP1 and PP2A. Nat Commun. 2020 Jul 17;11(1):3583.
http://www.nephrolab.org/groups/michael-koettgen
Methods: Cloning, Protein Expression and Purification, Establishing cell based library screening
https://laux.biologie.uni-freiburg.de/
Methods: Establishing cell sorting assay for plant cells
https://www.uniklinik-freiburg.de/iuk/forschungsgruppen/exosomen-und-tumorbiologie.html
Methods: Cloning, Stable cell line generation
Publications: In process
Methods: Cloning, Stable cell line generation (knock-out and over-expression)
https://www.bioss.uni-freiburg.de/prof-dr-michael-reth
Methods: Cloning, Large scale protein expression and purification
Methods: Protein expression, purification and refolding
Publications: In preparation
Methods: Cloning, Kinetic measurements (Octet RED96 System)
Publication: Comparison of different label-free imaging high-throughput biosensing systems for aptamer binding measurements using thrombin aptamers.
Anal Biochem. 2019 Oct 15;583:113323. doi: 10.1016/j.ab.2019.05.012
https://www.uniklinik-freiburg.de/pathologie/forschung/ag-schilling.html
Methods: Cloning, Cell line generation (over-expression / knock-out cell line)
Publication: Impact of DJ-1 and Helix 8 on the Proteome and Degradome of Neuron-Like Cells. Cells. 2021 Feb 16;10(2):404. doi: 10.3390/cells10020404.
Methods: Protein expression and purification, Stable cell line generation (over-expression / knock-out cell line), Cloning
Publication: Disordered Conformation with Low Pii Helix in Phosphoproteins Orchestrates Biomimetic Apatite Formation.
Sarem M, Lüdeke S, Thomann R, Salavei P, Zou Z, Habraken W, Masic A, Shastri VP.
Adv Mater. 2017 Sep;29(35). doi: 10.1002/adma.201701629
Methods: Cloning, Kinetic measurement (Octet RED96 System), Polarization measurement, Protein expression and purification, Stable cell line generation
Publications: Designed membrane protein heterodimers and control of their affinity by binding domain and membrane linker properties.
Lan C, Stulz A, Barthes N.P.F., Lauw S, Salavei P, Jung M, Heerklotzc H, and Ulbrich M.H.
Nanoscale 2021 Dec 16;13(48):20692-20702, doi: 10.1039/d1nr06574b.
https://www.uniklinik-freiburg.de/psych/team/vestring.html
Methods: qRT-PCR analysis, Western-Blot analysis
Publications:
https://www.bioss.uni-freiburg.de/groups/synthetic-biology/intro/
Methods: Large scale protein expression and purification
Publication: Production of Phytochromes by High-Cell-Density E. coli Fermentation ACS Synth Biol. 2019 Oct 18;8(10):2442-2450.doi: 10.1021/acssynbio.9b00267.