Instrumentations

Robotics Facility

Automation

 

 

 

 

 

 

Tecan Freedom Evo 200 Liquid Handling System with disposable tips

Robotic setup:

  • 8 chanel disposable tips, Pipetting: 1-1000 µl
  • Heated incubator to 37 °C without CO2
  • Carriers can be adjusted for single temperature (FL601 Recirculating Cooler: -20 - +40 °C)
  • Carousel with stacking system for 110 plates, 21 racks of disposable tips 10-200 µl and 12 racks of disposable tips 1000 µl
  • Biotek Microplate Washer 405TMTS

Biotek Microplate Washer 405TMTS for 95 and 384 well plate

System can be connected with:

  • Biotek Synergy H4 Hybrid reader
  • Biotek Multiflo Microplate Dispenser
  • Lightcycler®480 Real-time PCR

 

 

 

 

 

 

 

 

 

Biotek Synergy H4 Hybrid reader

96 well microplate/2 min

Detection:

  • Absorption: 96, 384, 1536 well plates
  • Fluorescence: 96 and 384 well plates
  • Luminescence: 96 and 384 well plates
  • FRET
  • TR-FRET
  • Fluorescence Polarization (Excitation: 360/40, 485/20, 540/25 and Emission: 460/40, 528/40, 620/40)

 

Biotek Multiflo Microplate Dispenser

The dispenser can be used for 96, 384 and 1536 microplates, using two different reagents. The machine can dispense 384 well plate in 6 seconds with minimum volume of 1 µl.

 

 

 

 

 

Tecan Freedom Evo 200 Liquid Handling System fixed tips

Robotic setup:

  • 8 chanel fixed  tips
  • CO2 incubator with carousel for 15 independent LED light boxes at 490, 660, 740 nm wavelength
  • Carriers can be adjusted for 2 different temperatures ( e.g., +4 and +37 °C)
  • Hettich centrifuge for microplates (Rotanta460 Robotic)
  • Flow cytometry for 96 well plate (Macsquant®  analyzer 10) 

 

 

 

Liconic Worktable Incubator:

  • 2 slots can run parallel
  • Temperature: RT or 37 °C
  • LED light: 490, 660, 740 nm
  • No connection for CO2

Liconic CO2 incubator with LED:

  • 15 slots can run parallel with different light programs
  • Temperature: 37 °C
  • LED light: 490, 660, 740 nm
  • Connection with CO2

Miltenyi MACSQuant Analyser 10

  • Lasers: 405,488,638 nm
  • 10 optical channels (two scatter (FSC, SSC) and eight fluorescent channels) for multi-parameter flow cytometry
  • Multi-sample analysis of up to 96 wells
  • Integrated auto-labeling and MACSQuant column for magnetic rare cell pre-enrichment

Measurement and Analysis

 

 

 

 

 

 

 

These are the machines that are accessible for the users:

BioTek EL406 Washer Dispenser

Applications for the EL406 Washer Dispenser run from simple dispense operations to full-scale automation of liquid handling steps in preparation for downstream processes.

Combination of Washer Dispenser (BioTek EL406), Plate stacker (Thermo Scientific RapidSTAK model F01490) and Plate reader (Synergy H4)

  • Combination of 4 washing buffers and 3 dispenser reagents
  • Compatible with 96,384 and 1536 well plate
  • Automated for washing dispensing and transport to plate reader
  • Plate stacker up to 30 microplates

 

 

Octet RED96 System (FortéBio)

For 96-well characterization of protein-protein and protein-small molecule binding kinetics, and for the determination of protein concentrations and titer.

The Octet RED96 system uses disposable biosensors in an 8x12 array in a simple, Dip and Read™ label-free assay format to measure the titer of 96 samples in 30 minutes.