Metabolomics

LC-ESI QqQ Mass Spectrometry (Agilent 6460)

• Quantification of metabolites
• Systematic search on structurally homologous connections
• Elucidation of metabolic pathways
• Detection of minor metabolites in complex mixtures
• Semiquantitative determination of substance classes
• Control of labelling experiments
• Metabolic flux analysis

 

will be replaced by a new machine this year

 

 

LC-QqTOF Mass Spectrometry (Agilent 6520A)

• Metabolic profiling: database search of exact mass metabolites (Metlin Database)
• Structural analysis of unknown compounds
• Modelling of metabolic pathways
• Identification of metabolite modifications

 

will be replaced by a new machine this year

 

 

 

 

II. GC-EI-MS coupling (Agilent 7890A / 5975C) for metabolic profiling in plants, animals and microorganisms

We routinely profile the relative concentration changes in primary metabolites (sugars, sugar alcohols, amino acids, organic acids and phosphorylated compounds). The mainly polar metabolites are extracted from the tissue and derivatised, after which and the derivates are identified and quantified with an Agilent GC-Quadrupole-MS System, which can operate in electron ionization (EI) and chemical ionization (CI) modes.

Several more targeted methods for analyzing particular (i.e. non-polar) compounds are developed in cooperation with the various users.