The Life Imaging Center (LIC) is a central core facility of the Albert-Ludwigs-University Freiburg open to members of all Faculties. Currently 250 active users from 6 Faculties (Biology; Medicine; Chemistry and Pharmacy; Mathematics and Physics; Environment and Natural Resources; and Engineering) are using the infrastructure of the LIC. The users come from app. 84 workgroups and the usage time of the microscopes in 2017 was about 19.000 hours. We have now approximately 60 - 80 new users per year and the same number leaving the facility. Outside users from Freiburg (Max Planck Institutes, Fraunhofer Gesellschaft) and from other universities are also welcome. For the details of application follow this link.
TheImaging Facility of the Signalhaus as part of the LIC provides a collection of advanced light microscopy equipment for imaging and analyzing the molecular organization of cells.
The instruments in the Imaging Facility offer capabilities in wide-field fluorescence imaging and confocal microscopy. We are also equipped to perform live-cell imaging as well as measurements of fluorescence resonance energy transfer (FRET), total internal reflection fluorescence (TIRF), fluorescence lifetime (FLIM) and fluorescence correlation spectroscopy (FCS) experiments.
We assist through consultation, training and project collaborations. The facility is open to all members of the Signalhaus community as well as to external collaboration partners.
Techniques and Samples:
Most modern microscopic techniques (FRET, FRAP, FLIP, FLAP, photo-conversion, photo-activation, uncaging, spectral unmixing, multi-position and large area recording, TIRF, structured illumination, microinjection while imaging) can be performed in combination with live cells or organisms on the available microscope set-ups.
The LIC has a major focus on live cell imaging of all kind of cells, organisms and culture systems used in cell signaling research, developmental biology and neurobiology. Samples are: Embryos (Danio rerio, Drosophila melanogaster, Mus musculus, Xenopus laevis), model organisms or cell clusters (C.elegans, spheres, cysts), plants (root, leave, growth cone), neuronal cultures, explants and biopsies from tissue slices, spheroids, flow cultures as well as cells cultured on glass slides.
LIC is specialized in long time observation and experiments on these objects, high resolution large area and/or multiple location recordings of thick objects with 2P and UV/VIS excitation. A further specialty is culturing and permanent microscopic observation of cells and spheroids over periods of up to 10 days (also under flow conditions in special chambers).
All types of fluorescent proteins including many photo-activatable and photo-convertible are used. We have a large library of fluorescent proteins (more than 150) available for our users via the BIOSS toolbox and we perform test experiments with these constructs before giving them out to our customers. See also: Available excitation wavelengths for imaging at LIC (PDF).